A master mix of different capture antibodies coupled to BMBs is prepared for performing a BMB-based multiplex immunoassay. The sample is incubated with the mixed BMB pool. Since each bead can be distinguished based on its barcode, each can represent a capture antibody for a specific antigen or biomarker. After incubation with biotin labeled capture antibody mix, the presence of cytokine/chemokine are detected with Streptavidin-Phycoerythrin conjugate (SA-PE).
A BMB-based immunoassay can be performed in sandwich format for cytokine detection. In brief, we use barcodes to identify which antibody is on each bead and use fluorescence to quantify biomarkers.
