Digital Barcodes for Multiplex Immunoassay*

BMB-based Multiplex Immunoassay

A master mix of different capture antibodies coupled to BMBs is prepared for performing a BMB-based multiplex immunoassay. The sample is incubated with the mixed BMB pool. Since each bead can be distinguished based on its barcode, each can represent a capture antibody for a specific antigen or biomarker. After incubation with biotin labeled capture antibody mix, the presence of cytokine/chemokine are detected with Streptavidin-Phycoerythrin conjugate (SA-PE).

 

Multiplex BMB Immunoassay

Multiplex BMB-Immunoassay Protocol:

A BMB-based immunoassay can be performed in sandwich format for cytokine detection. In brief, we use barcodes to identify which antibody is on each bead and use fluorescence to quantify biomarkers.


For More Information:

For more information about the technology of any of these products or to discuss a potential study, please contact us via:


* Multiplex immunoassay products are intended For Research Use only. Not for use in Diagnostic Procedures.